Proteins play an integral role in human health. As such, basic, applied, and clinical research aim to understand how protein expression, localization, post translational modifications (PTMs), and interactions regulate homeostasis and disease (see yellow box). Our current understanding of how proteins affect cellular phenotype is based on numerous applications, such as affinity purification, western blotting, and in vitro functional assays. Many of these techniques employ recombinant proteins, or proteins expressed in exogenous host systems. In this blog, we will discuss 1) how recombinant proteins are made, 2) the advantages of using recombinant proteins in proteomics research, and 3) how recombinant proteins are used in various proteomics applications.
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